Regulatory

Part:BBa_K4611014

Designed by: Saiyu Luo   Group: iGEM23_CJUH-JLU-China   (2023-10-01)


T7 promoter

T7 promoter, which comes from T7 bacteriophage, is a strong promoter with specific response to T7 RNA Polymerase to initiate T7 phage gene transcription. T7 RNA polymerase is highly promoter-specific and can specifically transcribe DNA or DNA replicas located downstream of T7 promoter in T7 phage. T7 RNA polymerase can selectively activate the transcription of T7 phage promoter, and its synthesis rate of mRNA is about 5 times faster than that of RNA polymerase from E.coli. Consequently, in our project, we used the T7 promoter to initiate our transcription to efficiently express the protein or obtain crRNA.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None